Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:310-9 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.08.002
Food Microbiology
Biofilm formation by Staphylococcus aureus and Salmonella spp. under mono and dual-species conditions and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite
Maricarmen Iñiguez-Moreno, Melesio Gutiérrez-Lomelí, Pedro Javier Guerrero-Medina, María Guadalupe Avila-Novoa,
Universidad de Guadalajara, Centro Universitario de la Ciénega, Departamento de Ciencias Médicas y de la Vida, Ocotlán, Jalisco, Mexico
Received 26 January 2017, Accepted 09 August 2017
Abstract

The aim of this study was evaluated the biofilm formation by Staphylococcus aureus 4E and Salmonella spp. under mono and dual-species biofilms, onto stainless steel 316 (SS) and polypropylene B (PP), and their sensitivity to cetrimonium bromide, peracetic acid and sodium hypochlorite. The biofilms were developed by immersion of the surfaces in TSB by 10 d at 37°C. The results showed that in monospecies biofilms the type of surface not affected the cellular density (p>0.05). However, in dual-species biofilms on PP the adhesion of Salmonella spp. was favored, 7.61±0.13Log10CFU/cm2, compared with monospecies biofilms onto the same surface, 5.91±0.44Log10CFU/cm2 (p<0.05). The mono and dual-species biofilms were subjected to disinfection treatments; and the most effective disinfectant was peracetic acid (3500ppm), reducing by more than 5Log10CFU/cm2, while the least effective was cetrimonium bromide. In addition, S. aureus 4E and Salmonella spp. were more resistant to the disinfectants in mono than in dual-species biofilms (p<0.05). Therefore, the interspecies interactions between S. aureus 4E and Salmonella spp. had a negative effect on the antimicrobial resistance of each microorganism, compared with the monospecies biofilms.

Keywords
Biofilms, Salmonella spp., Staphylococcus aureus, Disinfectants, Sensitivity
Braz J Microbiol 2018;49:310-9 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.08.002