Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2016;47 Supl 1:51-63 - Vol. 47 DOI: 10.1016/j.bjm.2016.10.007
Biotechnology and Industry Microbiology
Biopharmaceuticals from microorganisms: from production to purification
Angela Faustino Jozalaa, Danilo Costa Geraldesb, Louise Lacalendola Tundisib, Valker de Araújo Feitosac, Carlos Alexandre Breyerd, Samuel Leite Cardosoe, Priscila Gava Mazzolaf, Laura de Oliveira-Nascimentof,g, Carlota de Oliveira Rangel-Yaguic, Pérola de Oliveira Magalhãesh, Marcos Antonio de Oliveirad, Adalberto Pessoac,,
a Universidade de Sorocaba (UNISO), Departamento de Tecnologia e Processo Ambiental, Sorocaba, SP, Brazil
b Universidade de Campinas (UNICAMP), Instituto de Biologia, Programa de Pós-Graduação em Biociências e Tecnologia de produtos bioativos, Campinas, SP, Brazil
c Universidade de São Paulo, Departamento de Bioquímica e Tecnologia Farmacêutica, São Paulo, SP, Brazil
d Universidade Estadual de São Paulo (UNESP), Instituto de Biociências, Campus do Litoral Paulista, SP, Brazil
e Universidade de Brasília, Faculdade de Ciências da Saúde, Programa de Pós-Graduação em Ciências Farmacêuticas, Brasília, DF, Brazil
f Universidade Estadual de Campinas, Faculdade de Ciências Farmacêuticas, Campinas, SP, Brazil
g Universidade Estadual de Campinas, Instituto de Biologia, Departamento de Bioquímica e Biologia Tecidual, Campinas, SP, Brazil
h Universidade de Brasília, Faculdade de Ciências da Saúde, Departamento de Farmácia, Brasília, DF, Brazil
Received 06 September 2016, Accepted 22 September 2016
Abstract

The use of biopharmaceuticals dates from the 19th century and within 5–10 years, up to 50% of all drugs in development will be biopharmaceuticals. In the 1980s, the biopharmaceutical industry experienced a significant growth in the production and approval of recombinant proteins such as interferons (IFN α, β, and γ) and growth hormones. The production of biopharmaceuticals, known as bioprocess, involves a wide range of techniques. In this review, we discuss the technology involved in the bioprocess and describe the available strategies and main advances in microbial fermentation and purification process to obtain biopharmaceuticals.

Keywords
Biopharmaceuticals, Fermentation process, Biotechnology, Upstream process, Downstream process
Braz J Microbiol 2016;47 Supl 1:51-63 - Vol. 47 DOI: 10.1016/j.bjm.2016.10.007