Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:872-8 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.010
Biotechnology and Industrial Microbiology
Carotenoid production in sugarcane juice and synthetic media supplemented with nutrients by Rhodotorula rubra l02
Mariana de Paula Bonadio, Lidyane Aline de Freita, , Márcia Justino Rossini Mutton
Faculdades de Ciências Agrárias e Veterinárias, Campus de Jaboticabal, Departamento de Tecnologia, Jaboticabal, SP, Brazil
Received 20 July 2017, Accepted 28 February 2018

In order for the use of biological carotenoids to become feasible, it is necessary to have adequate low cost sources and improved methods of cultivation. The aim of this study was to evaluate the effect of supplementation with nitrogen, phosphorus, zinc, and magnesium, on the biomass and carotenoid volumetric production by yeast Rhodotorula rubra L02 using a complex medium (sugarcane juice) and synthetic media (sucrose and maltose) as substrates. The experimental design used for each substrate was randomized in blocks with 16 treatments and 3 repetitions. The treatments were compound for 15 different combinations of nutrients (N; Mg; Zn; P, N+Mg; N+Zn; N+P; Mg+Zn; Mg+P; Zn+P; N+P+Zn; N+P+Mg; N+Zn+Mg; P+Zn+Mg; N+Zn+Mg+P) alone and combined, and a control. The results were submitted to analysis of variance and Tukey test at 5% significance level. Among the treatments evaluated, the highest production of dry biomass, with both maltose and sucrose, was observed for Mg (1.60g/L and 1.94g/L, respectively). Additionally, another treatment that stood out in terms of biomass production was the control treatment with maltose (1.54g/L). After the incubation time, killer activity was not observed since there was no formation of inhibition halo around the L02 yeast.

Yeasts, Pigments, Macronutrients, Micronutrients, Killer activity
Braz J Microbiol 2018;49:872-8 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.010