Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:909-13 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.011
Clinical Microbiology
Comparison of GeneXpert MTB/RIF assay and LED-FM microscopy for the diagnosis of extra pulmonary tuberculosis in Khyber Pakhtunkhwa, Pakistan
Anwar Sheed Khana,, , Sajid Alib, Muhammad Tahir Khand, Sajjad Ahmeda, Yasir Khattakc, Abduljabbarf, Muhammad Irfang, Wasim Sajjade
a Khyber Medical University Peshawar, Peshawar, Pakistan
b Provincial TB Reference Laboratory, Peshawar, Pakistan
c University of Peshawar, Centre for Biotechnology and Microbiology, Peshawar, Pakistan
d Capital University of Science & Technology, Islamabad, Pakistan
e Department of Biogenetics, National University of Medical Sciences, Rawalpindi, Punjab, Pakistan
f University of Haripur, Department of Medical Lab Technology, Haripur, Pakistan
g Sarhad University of Science and Information Technology, Institute of Biological Sciences, Peshawar, Pakistan
Received 04 April 2017, Accepted 28 February 2018
Abstract

GeneXpert is one of the recent technological instruments used to diagnose tuberculosis in a short span of time. In this study, the performance of GeneXpert MTB/RIF assay for the diagnosis of extrapulmonary tuberculosis (EPTB) was compared with light-emitting diode Fluorescent Microscopy (LED-FM) in Khyber Pakhtunkhwa, Pakistan. A total of 737 EPTB samples were collected from tuberculosis (TB) suspected patients. Out of these samples, male to female ratio was 53% (n=390) to 47% (n=347) respectively. The sensitivity and specificity was 73% and 100% for GeneXpert, while 40% and 100% for LED-FM microscopy. This shows that the sensitivity of GeneXpert is 40–50%, higher than LED-FM microscopy. GeneXpert also detected low number of bacilli as compared to LED-FM microscopy.

Keywords
GeneXpert, LED-FM microscopy, Sensitivity, Specificity
Braz J Microbiol 2018;49:909-13 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.011