Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:714-22 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2017.12.013
Environmental Microbiology
Cultivated bacterial diversity associated with the carnivorous plant Utricularia breviscapa (Lentibulariaceae) from floodplains in Brazil
Felipe Rezende Limaa,b, Almir José Ferreiraa,b, Cristine Gobbo Menezesc, Vitor Fernandes Oliveira Mirandac, Manuella Nóbrega Douradoa,, , Welington Luiz Araújoa,b,,
a Departmento de Microbiologia, Instituto de Ciencias Biomedicas, Universidade de São Paulo, Av. Prof. Lineu Prestes, 1374-Ed. Biomédicas II, Cidade Universitária, 05508-900 São Paulo, SP, Brazil
b Núcleo Integrado de Biotecnologia, NIB, Universidade de Mogi das Cruzes, Av. Dr. Cândido Xavier de Almeida Souza, 200, Centro cívico, 08780-911 Mogi das Cruzes, SP, Brazil
c Faculdade de Ciências Agrárias e Veterinárias, Universidade Estadual Paulista UNESP, Via de acesso Prof. Paulo Donato Castellane s/n, Centro, 14884-900 Jaboticabal, SP, Brazil
Received 18 September 2017, Accepted 24 December 2017
Abstract

Carnivorous plant species, such as Utricularia spp., capture and digest prey. This digestion can occur through the secretion of plant digestive enzymes and/or by bacterial digestive enzymes. To comprehend the physiological mechanisms of carnivorous plants, it is essential to understand the microbial diversity related to these plants. Therefore, in the present study, we isolated and classified bacteria from different organs of Utricularia breviscapa (stolons and utricles) and from different geographic locations (São Paulo and Mato Grosso). We were able to build the first bacterium collection for U. breviscapa and study the diversity of cultivable bacteria. The results show that U. breviscapa bacterial diversity varied according to the geographic isolation site (São Paulo and Mato Grosso) but not the analyzed organs (utricle and stolon). We reported that six genera were common to both sample sites (São Paulo and Mato Grosso). These genera have previously been reported to be beneficial to plants, as well as related to the bioremediation process, showing that these isolates present great biotechnological and agricultural potential. This is the first report of an Acidobacteria isolated from U. breviscapa. The role of these bacteria inside the plant must be further investigated in order to understand their population dynamics within the host.

Keywords
Utricularia breviscapa, Microbial ecology, Aquatic microbiota, Microbial communities
Introduction

There are over 700 reported species of carnivorous plants distributed in 5 orders (Caryophyllales, Ericales, Lamiales, Oxalidales and Poales) and 10 families that occur in different habitats all over the world.1 This carnivorous syndrome has arisen independently at least six times in the evolutionary history of angiosperms over an extensive evolutionary timespan and involving different morphological adaptations to capture and digest the prey.2,3

The plant must present certain features to be classified as carnivorous, such as actively attracting, capturing and digesting prey, absorbing nutrients from it and obtaining some advantages in growth or reproduction.4 The prey can be an additional source of N, P, S, K and Mg and complement these photoautotrophic plants.5,6

The genus Utricularia (Lentibulariaceae), with over 220 species, is the most widespread of the carnivorous plants. One species of this genus is Utricularia breviscapa, which can be naturally found in the Antilles and South America. It is an aquatic plant, and its suspended structures contain air-filled parenchyma that allows it to float. Its stolons are threadlike, and bear segmented leaves and utricules (the traps).7Utricularia species present foliar structures called utricles, which are highly specialized traps that are capable of capturing prey by suction through the action of negative internal hydrostatic pressure.8,9

In many carnivorous plant species, digestion does not necessarily occur through the secretion of plant digestive enzymes but is performed by bacterial and/or fungal digestive enzymes.2,10 For example, in some species of carnivorous plants, such as Byblis (Byblidaceae), Brocchinia (Bromeliaceae), Darlingtonia, Heliamphora and some species of Sarracenia (Sarraceniaceae), bacterial enzymes are essential in the absence of plant enzyme secretion, and among the plants that have such glands, the bacterial community also plays an important role in the optimization process of prey degradation.4,11

These endophytes living inside the plant have innumerous advantages, since the internal tissues provide a stable environment without ultraviolet rays, temperature fluctuations or nutrient competition with other microorganisms as well as an increased availability of nutrients.12–14 This microorganism-plant interaction is important for the survival of the plant, since these microorganisms represent several benefits for the host plant, providing protection against pathogens, promoting growth, increasing the ability to capture trace elements and several other benefits that are extremely important for the maintenance of plant balance.15,16

Therefore, it is important to understand the microbial diversity related to carnivorous plants, particularly bacterial diversity, since it can represent approximately 58% of the total viable microbial biomass,17 playing a key role in the trap. Caravieri et al.18 were the first to report the total bacterial diversity from Utricularia hydrocarpa and Genlisea filiformis traps using a non-cultivable approach, showing that only 1.2% of the observed operational taxonomic units (OTU) were shared by both analyzed plants (U. hydrocarpa and G. filiformis). Moreover, in addition to plant genotype, trap age can also influence the microbial communities inside the vesicles.19

The present study aims to isolate the bacterial community from different organs (stolons and utricles) of U. breviscapa, which may have great biotechnological potential, in order to build the first bacterium collection for U. breviscapa and study the diversity of cultivable bacteria, aiming to understand their function in this unique environment.

Materials and methodsPlant sampling

The samples of U. breviscapa plants were collected from two different floodplains in Brazil: (1) Santo Antônio de Leverger municipality, Mato Grosso state (MT) and (2) Rio Tietê flood plain, Mogi das Cruzes municipality, São Paulo state (SP) (Table 1 and Fig. 1). The sample plants were stored in plastic bags duly identified with sample number and the point from which they were collected, maintained in freshwater at environmental temperature. After collection, the samples were transported to the Laboratory of Molecular Biology and Microbial Ecology where the isolation was immediately carried out. Vouchers are deposited in Herbarium HUMC.

Table 1.

Sampling locations and characteristics.

General location  City  Coordinates  River/lake  Characteristics 
Mato Grosso state  Santo Antônio de Leverger  S15°59′31.8″ W55°48′57.4″  River Aricá floodplain  Lentic environment, low depth 
São Paulo state  Mogi das Cruzes  S23°31′58.5″ W46°08′38.3″  River Tietê floodplain  Lentic environment, medium depth 
Fig. 1.
(0.29MB).

Habit of Utricularia breviscapa with inflorescence (bar=10mm). The detail shows a stolon with utricles (arrow indicates an utricule; bar=5mm).

Bacterial isolation

The maceration method was adapted from Kuklinsky-Sobral et al.20 for the bacterial isolation from weighed stolons and utricles of U. breviscapa previously washed in sterilized water. Bacteria were isolated by soaking 5 stolon fragments (approximately 5mm per fragment) or 10 utricles in phosphate-buffered saline (PBS) containing Na2HPO4 (1.44gL−1), KH2PO4 (0.24gL−1), KCl (0.20gL−1), and NaCl (8.00gL−1), adjusting the pH to 7.4, and macerating. After maceration, the sample volume was adjusted to 1mL, appropriate dilutions were carried out and plated onto 10% trypticase soy agar (TSA) supplemented with 50mgmL−1 of the fungicide Imazalil (Magnate 500 CE, Agricur), and the plates were incubated at 28°C for 2–15 days. The colonies were removed from the plates, inoculated onto 10% TSA agar medium, incubated at 28°C for 2–10 days, and then each isolate was suspended in a 20% glycerol solution and stored at −70°C.

Statistical analyses were carried out with biological triplicates for each treatment for bacterial quantification in the isolation experiments, which were performed in a completely random design. The significance of the observed differences was verified using a new analysis of variance (p=0.05). Analyses were conducted using R software version 3.0.1.

Polymerase chain reaction (PCR) amplification and 16S rDNA sequencing

After cultivation, the bacterial DNA was extracted according to Araújo et al.21. A partial sequence of the 16S rRNA gene was amplified using the pair of primers R137822 and P027F.23 PCRs were performed according to Dourado et al.24 All PCR amplification was checked through electrophoresis on agarose gel (1.5%, w/v agarose) and UV visualization of the ethidium bromide-stained gels, after which the PCR products were purified using a GFX PCR DNA and gel band purification kit (Amersham Biosciences) and sequenced by Sanger Sequencing Technology25 using the primer 1378R.

Analysis of the 16S rRNA gene sequence

Sequences were obtained from the bacteria isolated from U. breviscapa stolons and utricles from São Paulo and Mato Grosso state. Prior to the analysis, all chromatograms were trimmed with Phred-Phrap (http://www.phrap.com/phred/). The sequences were clustered as operational taxonomic units (OTU) using MOTHUR26 and a cut-off of 97% identity and further examined using rarefaction analysis and Libshuff, and dendrograms were constructed. Furthermore, richness and diversity were also calculated using MOTHUR based on non-parametric richness (Ace and Chao1) and diversity (Shannon-Weaver and Simpson) indexes with cut-offs for similarity at 97% (0.03), 95% (0.05) and 91% (0.09) identity. Taxonomic classifications were performed using RDP Query (https://rdp.cme.msu.edu) as database parameters. For phenetic analysis, the sequences were aligned using ClustalW,27 and the distance was calculated using the Jukes and Cantor model28 using Neighbor Joining method in MEGA 5 software.29 The branches were tested with bootstrap analyses (1000 replications), and the layout of trees was designed using the online application “Interactive Tree Of Life” (iTOL) (http://itol.embl.de/).30

A total of 200 DNA sequences of partial 16S rRNA genes were deposited in the GenBank database under accession numbers KY453794 to KY453980.

Results and discussion

A total of 200 sequences were obtained from bacteria isolated from U. breviscapa. Higher isolated bacterial density was observed in association with plants from Mato Grosso (MT) (Fig. 2). The bacterial density averages from MT samples were 4.9×105CFUutricle−1 and 3.6×107CFUg of stolon−1, while the samples from São Paulo (SP) had bacterial densities of 6.0×104CFUutricle−1 and 1.2×107CFUg of stolon−1 (Fig. 2).

Fig. 2.
(0.11MB).

Bacterial density in (A) utricles and (B) stolons of U. breviscapa from Mato Grosso (MT) and São Paulo (SP). Statistically different at p<0.05.

The richness estimator of OTUs performed with the Chao1 and Ace indexes at 97% (Table 2) showed a greater bacterial richness in SP plants than in MT plants. According to the Chao1 parameter, we can observe the highest richness in utricles followed by stolons in SP plants. However, based on the Ace estimator, the opposite was observed, in which the Ace index showed the highest richness in stolons followed by utricles. In terms of bacterial richness in MT plants, Chao1 estimates a statistically equal richness in both utricles and stolons (Table 2).

Table 2.

Diversity index and richness estimation of analyzed OTUs.

Library  Similarity  OTUs  Chao-1  Ace  Shannon (H)  Simpson (1-D) 
      Richness estimatorDiversity indexes
utricSP  97%  37  107 (62–236)  104 (63–211)  3.2 (3.0–3.5)  0.95 (0.98–0.93) 
utricMT  97%  25  56 (34–124)  66 (38–152)  2.5 (2.1–2.9)  0.85 (0.94–0.76) 
stolSP  97%  34  93 (55–196)  228 (144–373)  3.3 (3.1–3.6)  0.97 (0.99–0.95) 
stolMT  97%  17  56 (27–168)  82 (46–163)  2.64 (2.3–2.9)  0.94 (0.99–0.90) 

It is common knowledge that the performance of non-parametric estimators depends on the species abundance distribution in the sample, and the preference for one over another is a difficult issue. Basualdo31 observed in his work that the Ace index was better when the observed richness was low and Chao1 when the observed richness was high. In another study, Hortal et al.32 mentioned that abundance-based non-parametric estimators (Ace and Chao1) are more precise compared with other richness estimators; however, their precision diminishes at lower sampling intensities, producing less consistent results. This suggests that the choice among richness estimator parameters can vary between sampling characteristics, even in the same study.

According to the Simpson index diversity at 97%, a higher diversity of OTUs was found in libraries constructed from SP plants, with the highest diversity in stolons (stolSP) followed by utricles (utricSP). Similar to São Paulo, the bacterial diversity index in MT plants was also higher in stolons (stolMT) followed by utricles (utricMT). The Shannon diversity index at 97% showed the same result.

Therefore, for all indexes used (Chao1, Ace, Simpson and Shannon), higher diversity rates were observed in plants from São Paulo. There is no apparent reason why São Paulo samples present a higher diversity when compared with Mato Grosso samples, but it is known that there are various characteristics that could be considered to contribute to this variation, of which the trap age has been the most cited in the literature, but the quality of water can also impact the microbial diversity. Plachno et al.19 found that old traps were colonized by attached bacteria, but they can also be found freely suspended in trap fluid, as shown in Sirová et al.17 However, determining utricle dynamics is further complicated by the rapid aging of traps (or pitcher), as their life cycle is completed over approximately 30 days, and many environmental changes occur during this time.33 Although their life cycle is short, the interior of utricles presents low concentrations of oxygen, and it has been postulated that the organisms trapped by Utricularia die as a result of oxygen depletion.34 This anoxic environment is reflected in its commensal communities, selecting for anaerobic and facultatively aerobic bacteria.35 Pitsch et al.36 reported a ciliate commensal that uses algae to circumvent the low concentration of oxygen inside the utricles. Furthermore, some researchers have assumed that this high abundance of commensal organisms also occurs in empty traps; in the same way, these microorganisms are not specialized for living inside the traps, and they can therefore live either on the external surface or freely as plankton in the ambient water.37

Another factor that could influence the microbial community inside the traps is the composition of the water surrounding the trap that will be sucked in together with the prey or even attached to the prey. Thus, the bacterial community is selected according to the physical–chemical conditions inside the trap. It is important to emphasize that the two sampled sites were located in floodplain areas. River floodplain systems are known for their heterogeneity in habitats and hydrological pulses, presenting great temporal and spatial variation in their limnological conditions, which, in turn, should influence the bacterial community composition.38

The Libshuff significance test indicated that those libraries belonging to the same part of the plant (stolSP/stolMT and utricSP/utricMT) (Table 3) differ significantly for each sampled site (São Paulo and Mato Grosso). Moreover, although they are associated with the same site, the significance analysis for utricleMT and stolonMT shows a significant difference between these parts of the host plant. On the other hand, when comparing utricleSP and stolonSP, which present higher richness and diversity in their 16S rDNA libraries, do not vary, indicating that only in less diverse environments are there different colonization patterns between stolons and utricles.

Table 3.

Libshuff analysis comparing coverage between libraries.

Comparison  Score  Significance 
stolMT-stolSP  0.006  0.0044 
stolSP-stolMT  0.025  <0.0001 
stolMT-utricMT  0.010  0.0015 
utricMT-stolMT  0.053  <0.0001 
stolMT-utricSP  0.011  0.0037 
utricSP-stolMT  0.069  <0.0001 
stolSP-utricMT  0.029  <0.0001 
utricMT-stolSP  0.009  <0.0001 
stolSP-utricSP  0.001  0.5787 
utricSP-stolSP  0.006  0.0009 
utricMT-utricSP  0.005  0.0001 
utricSP-utricMT  0.048  <0.0001 

A dendrogram of bacterial communities showed similarities between the same site libraries (Fig. 3). Agreeing with the richness and diversity indexes, the libraries were grouped according to the sampled site, grouping utricles and stolons from São Paulo (utricSP and stolSP) in the same branch (with presents higher diversity), while utricles and stolons from Mato Grosso (utricMT and stolMT) were grouped in another branch (which presents lower diversity). This indicates that there is greater bacterial community similarity between different parts of the same host plant collected from the same sampled site (São Paulo or Mato Grosso), corroborating the hypothesis that the place of origin plays the main role in bacteria-plant interactions, since the environment supplies the microbial diversity to the plant host.

Fig. 3.
(0.03MB).

Dendrogram of bacterial communities associated to U. breviscapa.

In looking at the 200 isolates from SP and MT, respectively, the 16S rRNA analysis of plant isolates from MT showed 22 bacterial genera, among them Sphingomonas (36.71%), Aquitalea (18.99%) and Bacillus (6.33%), while in the plant isolates from São Paulo, we found 31 bacterial genera, of which the predominant genera were Microbacterium (20.66%), Sphingomonas (14.05%) and Pelomonas (9.09%) (Fig. 4). Caravieri et al.18 also found Sphingomonas and Microbacterium genera in association with U. hydrocarpa, but the culture-independent methods used in their work found these OTUs among the less abundant genera.

Fig. 4.
(0.83MB).

Phenetic trees representing the cultivable bacterial found in utricles and stolons from U. breviscapa were built using Neighbor Joining method in MEGA 5 software. (A) Isolates from Mato Grosso and (B) from São Paulo. The solid gray circles next to the tree branches correspond to bootstrap values higher than 50%. Brachyspira innocens (S000437178); Brachyspira hyodysenteriae (S000437188), Brachyspira hyodysenteriae (S000437188) and Brachyspira hyodysenteriae (S000437188) was used as outgroup.

Six genera were common to both sampled sites (SP and MT): Aquitalea, Azospirillum, Bacillus, Chromobacterium, Novosphingobium and Sphingomonas (Figs. 4 and 5). As shown for bacterial density, it was also verified that the abundance of cultivable bacterial species varied according to the sampled sites, suggesting that the plant selects the bacterial community in its tissues, and this selection must occur from the diversity present in its surrounding environment. Moreover, Koopman et al.39 observed that the bacterial community within the traps of Sarracenia alata plants was significantly different from the soil surrounding the plant and varied over time, suggesting that the community associated with the plants should not occur randomly but should be dependent on the interaction between the plant genotype and the environmental conditions.

Fig. 5.
(0.08MB).

Venn diagram represented by shared UTO's of stolons (stol) and utricules (utric) of U. breviscapa from Mato Grosso (MT) and São Paulo (SP) (dissimilarity of 0.09). Therefore: utricMT: utricules from Mato Grosso, stolSP: stolons from São Paulo, utricSP: utricules from São Paulo and stolMT: stolons from Mato Grosso.

The Utricularia samples were taken from muddy and dirty water, where the most abundant genera of both studied sites belonged to the Sphingomonas genus, which has been previously reported to colonize different polluted environments, both aquatic and terrestrial, being able to use polycyclic aromatic hydrocarbons as its sole source of carbon and energy.40 Moreover, this genus has been reported to have a beneficial interaction with plants, producing phytohormones such as gibberellins and indole acetic acid, promoting the growth of Solanum lycopersicum,41 and producing exopolysaccharides,42,43 which have also been reported to be a factor in plant–bacterium interactions for Paenibacillus polymyxa.44

In the present work, the genus Aquitalea was one of the most abundant genera associated with utricles from sampled SP and MT Utricularia. The genus Aquitalea was described in 2006 by Lau et al.,45 from a facultative anaerobic bacterium strain living in lakes. This genus is most commonly found in humic and oligotrophic lakes and in other water sources.46 Woo et al.47 showed that the genus Aquitalea was one of the most abundant genera isolated from wet tropical forest soils, an environment rich in organic matter to decompose, presenting high levels of enzyme activities. Although little is known about the mechanism of digestion in Utricularia, evidence has been raised for the presence of enzymatic activity inside the trap, and a considerable proportion of the enzymatic activity in the trap fluid is assumed to be derived from the bacterial community.48

Other abundant genera associated with Utricularia from SP were Pelomonas and Microbacterium, which have also been reported as beneficial bacteria to host plants. Pelomonas spp. isolated from industrial water or from humic and oligotrophic lakes are able to fix nitrogen and reduce nitrate45,49; they can also be involved in the bioremediation process because they degrade aromatic compounds. Microbacterium isolated endophytically and from the plant rhizoplane are also able to fix nitrogen,50 solubilize phosphate, oxidize sulphur, reduce nitrate and produce both indole acetic acid (IAA) and ACC deaminase (associated with stress reduction in plants by the inhibition of ethylene synthesis), showing their potential to promote plant growth.51

The Bacillus spp. found in both libraries (SP and MT) also have plant beneficial characteristics, such as the presence of the nitrate reductase enzyme and the potential to inhibit several phytopathogenic fungi (for example, Alternaria alternata, Cryphonectria parasitica, Fusarium graminearum, Phytophthora capsici and Rhizoctonia solani), demonstrating their important role in host plant defense.52

Furthermore, SP plants presented exclusive groups, such as the Actinobacteria that were isolated from stolons and utricles, confirming the specificity dependent on the geographic location from which the plants were collected. However, despite presenting similar libraries with no difference in the Libshuff significance test, plant organs can also select a bacterial group, such as the phyla Acidobacteria and Bacteroidetes, which were found only in utricles from plants collected in São Paulo and Mato Grosso, respectively (Fig. 4). These results support the hypothesis that plants select the bacterial communities associated with stolons and utricles based on the available community present in their surrounding environment.

Another important point we need to address is the utricle isolates that belong to the Acidobacteria group, identified as the genus Terriglobus albidus (99% similarity), a genus of fastidious growth (Fig. 6). Phylum Acidobacteria is one of the most abundant in soils, and its members have been found by cultivation independent methods in many types of environments. Acidobacteria is known to be abundant in trap fluid, and some species are known to live in acidic environments.53 Sirová et al.17 showed that the trap fluid pH is low in U. vulgaris, ranging from pH 4.2 to 5.1 according to the trap age, and this could be correlated with the phosphatase activity inside the trap.

Fig. 6.
(0.88MB).

Phenetic tree representing the cultivable Acidobacteria found in association with utricles of U. breviscapa from SP using Neighbor Joining method in MEGA 5 software. Numbers above the branches indicates bootstrap values. Brachyspira species were used as outgroup.

However, members of the Acidobacteria group have proven difficult to isolate and cultivate under laboratory conditions, and there are still few species with valid names, and few are well defined; among them are members of the genera Acidobacterium, Acanthopleuribacter, Bryobacter, Edaphobacter, Geothrix, Granulicella, Holophaga and Terriglobus.54,55Terriglobus sp. was previously isolated from water56 and from specific soil conditions, such as desert soils,57 arctic tundra soils58 and the rhizosphere of a medicinal plant.59T. albidus was described in 201560 isolated from a semiarid savannah soil collected in northern Namibia, however the present work present the first report of cultivable bacteria belonging to this genus isolated in association with a carnivorous plant.

Caravieri et al.,18 using culture-independent methods, reported that Acidobacterium was one of the dominant genera in association with U. hydrocarpa. There are advantages in retrieving an Acidobacterium isolate due to its biotechnological applications, such as the production of an extracellular matrix that apparently facilitates the flocculation of cells in liquid media.61 Therefore, our research group is currently investigating the biotechnological potential of these Acidobacterium isolates.

Conclusions

The results show that the bacterial diversity of U. breviscapa varied according to the isolation location (São Paulo and Mato Grosso) rather than organs. This is also the first report of an Acidobacteria (Terriglobus genus) isolated in association with utricles of U. breviscapa. The role of these bacteria inside the utricles and stolons must be further investigated in order to understand their population dynamics within the host plant.

Conflicts of interest

Declarations of interest: none

References
1
E. Król,B.J. Płachno,L. Adamec,M. Stolarz,H. Dziubinska,K. Trebacz
Quite a few reasons for calling carnivores ‘the most wonderful plants in the world’
Ann Bot, 109 (2011), pp. 47-64 http://dx.doi.org/10.1093/aob/mcr249
2
A.M. Ellison,N.J. Gotelli
Energetics and the evolution of carnivorous plants-Darwin's ‘most wonderful plants in the world’
J Exp Bot, 60 (2009), pp. 19-42 http://dx.doi.org/10.1093/jxb/ern179
3
K. Reifenrath,I. Theisen,J. Schnitzler,S. Porembski,W. Barthlott
Trap architecture in carnivorous Utricularia (Lentibulariaceae)
Flora, 201 (2006), pp. 597-605
4
T.J. Givnish
New evidence on the origin of carnivorous plants
Proc Natl Acad Sci USA, 112 (2015), pp. 10-11 http://dx.doi.org/10.1073/pnas.1422278112
5
L. Adamec
Mineral nutrition of carnivorous plants
Bot Rev, 63 (1997), pp. 273-299
6
A.M. Ellison,N.J. Gotelli
Evolutionary ecology of carnivorous plants
Trends Ecol Evol, 16 (2001), pp. 623-629
7
P. Taylor
The genus Utricularia
A taxonomic monograph. Kew bulletin additional series XIV. Royal Botanic Gardens, Kew, (1989)pp. 668-671
8
S. Poppinga,L.E. Daber,A.S. Westermeier
Biomechanical analysis of prey capture in the carnivorous Southern bladderwort (Utricularia australis)
9
B.J. Płachno,L.L. Adamec,I.K. Lichtscheidl,M. Peroutka,W. Adlassnig,J. Vrba
Fluorescence labelling of phosphatase activity in digestive glands of carnivorous plants
Plant Biol, 8 (2006), pp. 813-820 http://dx.doi.org/10.1055/s-2006-924177
10
M.W. Chase,M.J.M. Christenhusz,D. Sanders,M.F. Fay
Murderous plants: Victorian Gothic, Darwin and modern insights into vegetable carnivory
Bot J Linn Soc, 161 (2009), pp. 329-356
11
W. Adlassnig,M. Peroutka,T. Lendl
Traps of carnivorous pitcher plants as a habitat: composition of the fluid, biodiversity and mutualistic activities
Ann Bot, 107 (2011), pp. 181-194 http://dx.doi.org/10.1093/aob/mcq238
12
S. Bordiec,S. Paquis,H. Lacroix
Comparative analysis of defence responses induced by the endophytic plant growth-promoting rhizobacterium Burkholderia phytofirmans strain PsJN and the non-host bacterium Pseudomonas syringae pv. pisi in grapevine cell suspensions
J Exp Bot, 62 (2011), pp. 595-603 http://dx.doi.org/10.1093/jxb/erq291
13
J.A. Mcinroy,J.W. Kloepper
Survey of indigenous bacterial endophytes from cotton and sweet corn
Plant Soil, 173 (1995), pp. 337-342
14
J.A. Mcinroy,J.W. Kloepper
Population dynamics of endophytic bacteria in field-grown sweet corn and cotton
Can J Microbiol, 41 (1995), pp. 895-901
15
R.P. Ryan,K. Germaine,A. Franks,D.J. Ryan,D.N. Dowling
Bacterial endophytes: recent developments and applications
FEMS Microbiol Lett, 278 (2008), pp. 1-9 http://dx.doi.org/10.1111/j.1574-6968.2007.00918.x
16
A. Sessitsch,M. Kuffner,P. Kidd
The role of plant-associated bacteria in the mobilization and phytoextraction of trace elements in contaminated soils
Soil Biol Biochem, 60 (2013), pp. 182-194 http://dx.doi.org/10.1016/j.soilbio.2013.01.012
17
D. Sirová,J. Borovec,B. Cerna,E. Rejmánková,L. Adamec
Microbial community development in the traps of aquatic Utricularia species
Aquat Bot, 90 (2009), pp. 129-136
18
F.A. Caravieri,A.J. Ferreira,A. Ferreira,D. Clivati,V.F.O. Miranda,W.L. Araújo
Bacterial community associated with traps of the carnivoral plants Utricularia hydrocarpa and Genlisea filiformis
Aquat Bot, 116 (2014), pp. 8-12
19
B. Płachno,M. Łukaszek,K. Wołowski,L. Adamec,P. Stolarczyk
Aging of Utricularia traps and variability of microorganisms associated with that micro-habitat
Aquat Bot, 97 (2012), pp. 44-48
20
J. Kuklinsky-Sobral,W.L. Araújo,R. Mendes,A.A. Pizzirani-Kleiner,J.L. Azevedo
Isolation and characterization of endophytic bacteria from soybean (Glycine max) grown in soil treated with glyphosate herbicide
Plant Soil, 273 (2005), pp. 91-99
21
W.L. Araújo,J. Marcon,W. Maccheroni Jr.,J.D. van Elsas,J.W. van Vuurde,J.L. Azevedo
Diversity of endophytic bacterial populations and their interaction with Xylella fastidiosa in citrus plants
Appl Environ Microbiol, 68 (2002), pp. 4906-4914
22
H. Heuer,M. Krsek,P. Baker,K. Smalla,E.M.H. Wellington
Analysis of actinomycete communities by specific amplification of genes encoding 16S rRNA and gel-electrophoretic separation in denaturing gradients
Appl Environ Microbiol, 63 (1997), pp. 3233-3241
23
D.J. Lane,B. Pace,G.J. Olsen,D.A. Stahl,M.L. Sogin,N.R. Pace
Rapid-determination of 16S ribosomal RNA sequences for phylogenetic analyses
Proc Natl Acad Sci USA, 82 (1985), pp. 6955-6959
24
M.N. Dourado,F.D. Andreote,F. Dini-Andreote,R. Conti,J.M. Araújo,W.L. Araújo
Analysis of 16S rRNA and mxaF genes revealing insights into Methylobacterium niche-specific plant association
Genet Mol Biol, 35 (2012), pp. 142-148
25
F. Sanger,S. Nicklen,A.R. Coulson
DNA sequencing with chain-terminating inhibitors
Proc Natl Acad Sci USA, 74 (1977), pp. 5463-5467
26
P.D. Schloss,S.L. Westcott,T. Ryabin
Introducing mothur: open-source, platform-independent, community-supported software for describing and comparing microbial communities
Appl Environ Microbiol, 75 (2009), pp. 7537-7541 http://dx.doi.org/10.1128/AEM.01541-09
27
J.D. Thompson,D.G. Higgins,T.J. Gibson
Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice
Nucl Acid Res, 22 (1994), pp. 4673-4680
28
T.H. Jukes,C.R. Cantor
Evolution of protein molecules
Mammalian protein metabolism, pp. 21-132
29
K. Tamura,D. Peterson,N. Peterson,G. Stecher,M. Nei,S. Kumar
MEGA5: molecular evolutionary genetics analysis using maximum likelihood, evolutionary distance, and maximum parsimony methods
Mol Biol Evol, 28 (2011), pp. 2731-2739 http://dx.doi.org/10.1093/molbev/msr121
30
I. Letunic,P. Bork
Interactive Tree Of Life (iTOL): an online tool for phylogenetic tree display and annotation
Bioinformatics, 23 (2007), pp. 127-128 http://dx.doi.org/10.1093/bioinformatics/btl529
31
C.V. Basualdo
Choosing the best non-parametric richness estimator for benthic macroinvertebrates databases
Rev Soc Entomol Argent, 70 (2011), pp. 27-38
32
J. Hortal,P.A. Borges,C. Gaspar
Evaluating the performance of species richness estimators: sensitivity to sample grain size
J Anim Ecol, 75 (2006), pp. 274-287
33
L.E. Friday
Rapid turnover of traps in Utricularia vulgaris L
Oecologia, 80 (1989), pp. 272-277 http://dx.doi.org/10.1007/BF00380163
34
B.J. Plachno
The carnivorous plants and Charles Darwin – a story about beautiful fascination
Karol Darwin w oczach polskich botaników XIX–XXI w,
35
L.D. Alcaraz,S. Martínez-Sánchez,I. Torres,E. Ibarra-Laciette,L. Herrera-Estrella
The metagenome of Ultricularia gibba's traps: into the microbial input to a carnivorous plant
PLOS ONE, 11 (2016), pp. 1-21
36
G. Pitsch,L. Adamec,S. Dirren
The green Tetrahymena utriculariae n. sp. (Ciliophora, Oligohymenophorea) with its endosymbiotic algae (Micractinium sp.), living in traps of a carnivorous aquatic plant
J Eukaryot Microbiol, 64 (2017), pp. 322-335 http://dx.doi.org/10.1111/jeu.12369
37
L. Adamec
The smallest but fastest. Ecophysiological characteristics of traps of aquatic carnivorous Ultricularia
Plant Signal Behav, 6 (2011), pp. 640-646
38
T. Laque,V.F. Farjalla,A.S. Rosado,F.A. Esteves
Spatio temporal variation of bacterial community composition and positive controlling factors in tropical shallow lagoons
Microb Ecol, 59 (2010), pp. 819-829 http://dx.doi.org/10.1007/s00248-010-9642-5
39
M.M. Koopman,D.M. Fuselier,S. Hird,B.C. Carstens
The carnivorous pale pitcher plant harbors diverse, distinct, and time-dependent bacterial communities
Appl Environ Microbiol, 76 (2010), pp. 1851-1860 http://dx.doi.org/10.1128/AEM.02440-09
40
N.M.E.J. Leys,A. Ryngaert,L. Bastiaens,W. Verstraete,E.M. Top,D. Springael
Occurrence and phylogenetic diversity of Sphingomonas strains in soils contaminated with polycyclic aromatic hydrocarbons
Appl Environ Microbiol, 70 (2004), pp. 1944-1955
41
A.L. Khan,M. Waqas,S. Kang
Bacterial endophyte Sphingomonas sp. LK11 produces gibberellins and IAA and promotes tomato plant growth
J Microbiol, 52 (2014), pp. 689-695 http://dx.doi.org/10.1007/s12275-014-4002-7
42
V.D. Prajapati,G.K. Jani,B.S. Zala,T.A. Khutliwala
An insight into the emerging exopolysaccharide gellan gum as a novel polymer
Carbohyd Polym, 93 (2013), pp. 670-678
43
E. Schultheis,M.A. Dreger,M. Nimtz,V. Wray,D.C. Hempel,B. Nörtemann
Structural characterization of the exopolysaccharide PS-EDIV from Sphingomonas pituitosas train DSM 13101
Appl Microbiol Biotechnol, 78 (2008), pp. 1017-1024 http://dx.doi.org/10.1007/s00253-008-1383-8
44
I.V. Yegorenkova,K.V. Tregubova,V.V. Ignatov
Paenibacillus polymyxa Rhizobacteria and their synthesized exoglycans in interaction with wheat roots colonization
Curr Microbiol, 66 (2013), pp. 481-486 http://dx.doi.org/10.1007/s00284-012-0297-y
45
H. Lau,J. Faryna,E.W. Triplett
Aquitale amagnusonii gen. nov., sp. nov., a novel Gram-negative bacterium isolated from a humic lake
Int J Syst Evol Microbiol, 56 (2006), pp. 867-871 http://dx.doi.org/10.1099/ijs.0.64089-0
46
J. Sedlacek,S.W. Kwon,P. Svec
Aquitalea pelogenes sp. nov., isolated from mineral peloid
Int J Syst Evol Microbiol, 66 (2015), pp. 962-967 http://dx.doi.org/10.1099/ijsem.0.000819
47
H.L. Woo,T.C. Hazen,B.A. Simmons,K.M. DeAngelis
Enzyme activities of anaerobic lignocellulolytic bacteria isolated from wet tropical forest soils
Syst Appl Microbiol, 37 (2010), pp. 60-67 http://dx.doi.org/10.1016/j.syapm.2013.10.001
48
D. Sirová,L. Adamec,J. Vrba
Enzymatic activities in traps of four aquatic species of the carnivorous genus Utricularia
New Phytol, 159 (2003), pp. 669-675
49
M. Gomila,B. Bowien,E. Falsen,E.R.B. Moore,J. Lalucat
Description of Pelomonas aquatica sp. nov. and Pelomonas puraquae sp. nov., isolated from industrial and haemodialysis water
Int J Syst Evol Microbiol, 57 (2007), pp. 2629-2635 http://dx.doi.org/10.1099/ijs.0.65149-0
50
F. Zakhia,H. Jeder,A. Willems,M. Gillis,B. Dreyfus,P. Lajudie
Diverse bacteria associated with root nodules of spontaneous legumes in Tunisia and first report for nifH-like gene within the genera Microbacterium and Starkeya
Microb Ecol, 51 (2006), pp. 375-393 http://dx.doi.org/10.1007/s00248-006-9025-0
51
M. Madhaiyan,S. Poonguzhali,J. Lee,K. Lee,V.S. Saravanan,P. Santhanakrishnan
Microbacterium azadirachtae sp. nov., a plant growth-promoting Actinobacterium isolated from the rhizoplane of neem seedlings
Int J Syst Evol Microbiol, 60 (2010), pp. 1687-1692 http://dx.doi.org/10.1099/ijs.0.015800-0
52
Z. Zhao,Q. Wanga,K. Wang,K. Brian,C. Liu,Y. Gu
Study of the antifungal activity of Bacillus vallismortis ZZ185 in vitro and identification of its antifungal components
Bioresour Technol, 101 (2010), pp. 292-297 http://dx.doi.org/10.1016/j.biortech.2009.07.071
53
Y. Takeuchi,S. Chaffron,M.M. Salcher
Bacterial diversity and composition in the luid of pitcher plants of the genus Nepenthes
Syst Appl Microbiol, 38 (2015), pp. 330-339 http://dx.doi.org/10.1016/j.syapm.2015.05.006
54
S.A. Eichorst,C.R. Kuske,T.M. Schmidt
Influence of plant polymers on the distribution and cultivation of bacteria in the phylum Acidobacteria
Appl Environ Microbiol, 77 (2010), pp. 586-596 http://dx.doi.org/10.1128/AEM.01080-10
55
K. Okamura,A. Kawai,T. Yamada,A. Hiraishi
Acidipilarosea gen. nov., sp. nov., an acidophilic chemoorganotrophic bacterium belonging to the phylum Acidobacteria
FEMS Microbiol Lett, 317 (2011), pp. 138-142 http://dx.doi.org/10.1111/j.1574-6968.2011.02224.x
56
K.S. Baik,J.S. Choi,J. Kwon
Terriglobus aquaticus sp. nov., isolated from an artificial reservoir
Int J Syst Evol Microbiol, 63 (2013), pp. 4744-4749 http://dx.doi.org/10.1099/ijs.0.050724-0
57
R.M. Abed,S. Al-Kindi,S. Al-Kharusi
Diversity of bacterial communities along a petroleum contamination gradient in desert soils
Microb Ecol, 69 (2015), pp. 95-105 http://dx.doi.org/10.1007/s00248-014-0475-5
58
S.R. Rawat,M.K. Männistö,Y. Bromberg,M.M. Häggblom
Comparative genomic and physiological analysis provides insights into the role of Acidobacteria in organic carbon utilization in Arctic tundra soils
FEMS Microbiol Ecol, 82 (2012), pp. 341-355 http://dx.doi.org/10.1111/j.1574-6941.2012.01381.x
59
K.S. Whang,J.C. Lee,H.R. Lee,S.I. Han,S.H. Chung
Terriglobustenax sp. nov., an exopolysaccharide-producing Acidobacterium isolated from rhizosphere soil of a medicinal plant
Int J Syst Evol Microbiol, 64 (2014), pp. 431-437 http://dx.doi.org/10.1099/ijs.0.053769-0
60
J. Pascual,P.K. Wust,A. Geppert,B.U. Foesel,K.J. Huber,J. Overmann
Terriglobus albidus sp. nov., a member of the family Acidobacteriaceae isolated from Namibian semiarid savannah soil
Int J Syst Evol Microbiol, 65 (2015), pp. 3297-3304 http://dx.doi.org/10.1099/ijsem.0.000411
61
S.A. Eichorst,J.A. Breznak,T.M. Schmidt
Isolation and characterization of soil bacteria that define Terriglobus gen. nov., in the phylum acidobacteria
Appl Environ Microbiol, 73 (2007), pp. 2708-2717 http://dx.doi.org/10.1128/AEM.02140-06
Corresponding authors at: Department of Microbiology, Institute of Biomedical Sciences, University of São Paulo, Av. Prof. Lineu Prestes, 1374-Ed. Biomédicas II, Cidade Universitária, 05508-900 São Paulo, SP, Brazil. (Welington Luiz Araújo wlaraujo@usp.br)
Copyright © 2018. Sociedade Brasileira de Microbiologia
Braz J Microbiol 2018;49:714-22 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2017.12.013
Cookies Policy
x
To improve our services and products, we use cookies (own or third parties authorized) to show advertising related to client preferences through the analyses of navigation customer behavior. Continuing navigation will be considered as acceptance of this use. You can change the settings or obtain more information by clicking here.