Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:329-35 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.07.004
Veterinary Microbiology
Diagnostics of tuberculosis and differentiation of nonspecific tuberculin reactions in animals
Sovetzhan Z. Basybekova, Marat B. Bazarbayeva, Bolat A. Yespembetovb, Assiya Mussaevaa, Serik G. Kanatbayevd, Kanapya M. Romashevc, Aigul K. Dossanovaa, Tokseiit A. Yelekeyeve, Elmira K. Akmatovae, Nazym S. Syrymb,,
a Kazakh Research Veterinary Institute, Gvardeiskiy, Kazakhstan
b Research Institute of Biological Safety Problems, Gvardeiskiy, Kazakhstan
c Kazakh National Agrarian University, Gvardeiskiy, Kazakhstan
d West Kazakhstan Agrarian Technical University, Uralsk city, Kazakhstan
e Kyrgyz Research Institute of Veterinary named after A. Duysheev, Bishkek, Kyrgyzstan
Received 02 August 2016, Accepted 17 July 2017

Tuberculosis is a serious disease of humans and animals, caused by bacteria of the Mycobacterium genus. This leads to complications in the life of the sick person, and subsequently to death. The cattle, who have been diagnosed with this bacterium, are usually sent to the slaughter, with the result that their livestock is reduced. Mycobacteriosis is also a disease, after determining which cattle are most often sent to slaughter. Such a reduction in livestock numbers has a negative effect on the economy. Of the 300 samples from the animals, 25 cultures of atypical bacteria responding to tuberculin were isolated. A series of tests – intravenous tuberculin test, ophthalmic test, palpebral test, “ZhAT” test, showed that most of the tuberculosis changes in cattle were found in regional lymph nodes more often than in internal organs. In healthy for tuberculosis cows, at the age of 4–9 years, seasonal nonspecific sensitivity to tuberculin is observed. Implementation of the developed express method of glutaraldehyde test on farms in healthy tuberculosis will speed up the diagnosis of tuberculosis and mycobacteriosis in animals that reacted to tuberculin and will exclude short-term nonspecific sensitization of their organism to tuberculin. The introduction of this methodology can be used to diagnose and clearly differentiate the diagnoses of “tuberculosis” and “mycobacteriosis” in cattle. This will cure part of the livestock and reduce the amount of slaughter.

Cattle, Atypical mycobacterium, Tuberculin skin test, Palpebral method, Immunization
Braz J Microbiol 2018;49:329-35 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.07.004