Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2016;47:587-96 - Vol. 47 Num.3 DOI: 10.1016/j.bjm.2016.04.010
Environmental Microbiology
Effect of plant growth-promoting bacteria on the growth and fructan production of Agave americana L.
Neyser De La Torre-Ruiza, Víctor Manuel Ruiz-Valdiviezob, Clara Ivette Rincón-Molinab, Martha Rodríguez-Mendiolaa, Carlos Arias-Castroa, Federico Antonio Gutiérrez-Micelib, Héctor Palomeque-Dominguezb, Reiner Rincón-Rosalesb,,
a Plant Biotechnology, DEPI Instituto Tecnológico de Tlajomulco, Carretera a San Miguel Cuyutlán, Tlajomulco de Zúñiga, Jalisco, Mexico
b Laboratory of Biotechnology, Instituto Tecnológico de Tuxtla Gutiérrez, Tuxtla Gutiérrez, Mexico
Received 04 June 2015, Accepted 11 February 2016
Abstract

The effect of plant growth-promoting bacteria inoculation on plant growth and the sugar content in Agave americana was assessed. The bacterial strains ACO-34A, ACO-40, and ACO-140, isolated from the A. americana rhizosphere, were selected for this study to evaluate their phenotypic and genotypic characteristics. The three bacterial strains were evaluated via plant inoculation assays, and Azospirillum brasilense Cd served as a control strain. Phylogenetic analysis based on the 16S rRNA gene showed that strains ACO-34A, ACO-40 and ACO-140 were Rhizobium daejeonense, Acinetobacter calcoaceticus and Pseudomonas mosselii, respectively. All of the strains were able to synthesize indole-3-acetic acid (IAA), solubilize phosphate, and had nitrogenase activity. Inoculation using the plant growth-promoting bacteria strains had a significant effect (p<0.05) on plant growth and the sugar content of A. americana, showing that these native plant growth-promoting bacteria are a practical, simple, and efficient alternative to promote the growth of agave plants with proper biological characteristics for agroindustrial and biotechnological use and to increase the sugar content in this agave species.

Keywords
Agave, Inoculation, Bacteria, Inulin, 16S rRNA
Braz J Microbiol 2016;47:587-96 - Vol. 47 Num.3 DOI: 10.1016/j.bjm.2016.04.010