Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:777-84 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.01.003
Veterinary Microbiology
Molecular characterization of feline calicivirus variants from multicat household and public animal shelter in Rio de Janeiro, Brazil
Joylson de Jesus Pereiraa,, , Natasha Baumworcelb, Júlia Monassa Fiorettid, Cinthya Fonseca Dominguesa, Laís Fernandes de Moraesb, Robson dos Santos Souza Marinhoa, Maria Clara Rodrigues Vieiraa,c, Ana Maria Viana Pintoa, Tatiana Xavier de Castroa
a Universidade Federal Fluminense, Instituto Biomédico, Departamento de Microbiologia e Parasitologia, Niterói, RJ, Brazil
b Universidade Federal Fluminense, Faculdade de Veterinária, Niterói, RJ, Brazil
c Universidade Federal Fluminense, Instituto de Biologia, Departamento de Biologia Celular e Molecular, Programa de Pós-graduação em Ciências e Biotecnologia, Niterói, RJ, Brazil
d Instituto Oswaldo Cruz – Fiocruz, Laboratório de Virologia Comparada e Ambiental, Rio de Janeiro, RJ, Brazil
Received 29 August 2017, Accepted 14 January 2018
Abstract

The aim of this study was to perform the molecular characterization of conserved and variable regions of feline calicivirus capsid genome in order to investigate the molecular diversity of variants in Brazilian cat population. Twenty-six conjunctival samples from cats living in five public short-term animal shelters and three multicat life-long households were analyzed. Fifteen cats had conjunctivitis, three had oral ulceration, eight had respiratory signs (cough, sneeze and nasal discharge) and nine were asymptomatic. Feline calicivirus were isolated in CRFK cells and characterized by reverse transcription PCR target to both conserved and variable regions of open reading frame 2. The amplicons obtained were sequenced. A phylogenetic analysis along with most of the prototypes available in GenBank database and an amino acid analysis were performed. Phylogenetic analysis based on both conserved and variable region revealed two clusters with an aLTR value of 1.00 and 0.98 respectively and the variants from this study belong to feline calicivirus genogroup I. No association between geographical distribution and/or clinical signs and clustering in phylogenetic tree was observed. The variants circulating in public short-term animal shelter demonstrated a high variability because of the relatively rapid turnover of carrier cats constantly introduced of multiple viruses into this location over time.

Keywords
Conjunctivitis, FCV genogroup, Household, Molecular characterization, Shelter
Braz J Microbiol 2018;49:777-84 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.01.003
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