Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:914-8 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.07.002
Clinical Microbiology
Performance of rapid tests for carbapenemase detection among Brazilian Enterobacteriaceae isolates
Lisiane Rech Pancottoa, Carolina Silva Nodaria, Franciéli Pedrotti Rozalesa,b, Tatiane Soldia, Carolina Gomes Siqueirac, Ana Lúcia Freitasc, Afonso Luís Bartha,b,c,,
a Hospital de Clínicas de Porto Alegre, Laboratório de Pesquisa em Resistência Bacteriana (LABRESIS), Porto Alegre, RS, Brazil
b Universidade Federal do Rio Grande do Sul, Programa de Pós-Graduação em Ciências Farmacêuticas, Porto Alegre, RS, Brazil
c Universidade Federal do Rio Grande do Sul, Faculdade de Farmácia, Porto Alegre, RS, Brazil
Received 09 April 2017, Accepted 07 July 2018
Abstract

The global emergence of carbapenemases led to the need of developing new methods for their rapid detection. The aim of this study was to evaluate the performance of the rapid tests for carbapenemase-producing and non-producing Enterobacteriaceae. Carbapenem non-susceptible Enterobacteriaceae from a surveillance study submitted to a multiplex real time PCR for carbapenemase detection were included in this study. The isolates were subjected to the rapid phenotypic tests Carba NP, Blue-Carba and Carbapenem Inactivation Method (CIM). A total of 83 carbapenemase-producing (43) and non-producing (40) isolates were included in the study. The sensitivity/specificity were 62.7%/97.5%, 95.3%/100%, and 74.4%/97.5% for Carba NP, Blue-Carba and CIM, respectively. Both Carba NP and Blue-Carba presented their final results after 75min of incubation; the final results for CIM were obtained only after 8h. Failure to detect OXA-370 carbapenemase was the main problem for Carba NP and CIM assays. As the Blue-Carba presented the highest sensitivity, it can be considered the best screening test. Conversely, CIM might be the easiest to perform, as it does not require special reagents. The early detection of carbapenemases aids to establish infection control measures and prevent carbapenemases to spread reducing the risk of healthcare associated infections and therapeutic failure.

Keywords
Carbapenemases, Rapid tests, Carba NP, Blue-Carba, CIM
Braz J Microbiol 2018;49:914-8 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.07.002