Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:885-90 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.002
Clinical Microbiology
Performance of “RESIST-3 O.K.N. K-SeT” immunochromatographic assay for the detection of OXA-48 like, KPC, and NDM carbapenemases in Klebsiella pneumoniae in Turkey
Pınar Sağıroğlua,b,, , Ufuk Hasdemira, Gülşen Altınkanat Gelmeza, Burak Aksua, Onur Karatunac, Güner Söyletira
a Marmara University School of Medicine, Department of Medical Microbiology, Istanbul, Turkey
b Private Melikgazi Hospital, Medical Microbiology, Kayseri, Turkey
c Acibadem University School of Medicine, Department of Medical Microbiology, Istanbul, Turkey
Received 26 April 2017, Accepted 05 February 2018
Abstract

In this study, the performance of the “RESIST-3 O.K.N. K-SeT” (Coris BioConcept, Gembloux, Belgium) immunochromatographic assay was evaluated in 132 Klebsiella pneumoniae comprising 102 carbapenem resistant and 30 carbapenem susceptible isolates. Genotypically known isolates of Gram negative bacteria (n=22) including various species were also tested by the assay as controls. The isolates tested by the immunochromatographic assay and also were run PCR for blaKPC, blaIMP, blaVIM, blaNDM, and blaOXA-48. The rates of blaNDM, blaOXA-48, and blaKPC in carbapenem resistant isolates were found at 52.9%, 39.2%, and 2.0%, respectively. Both blaNDM and blaOXA-48 were found in six (5.9%) isolates. The results of the assay showed 100% concordance with those obtained by PCR in 132K. pneumoniae. The agreement between the two methods was found to be identical at the isolate level. The assay also correctly detected all genotypically known isolates of Escherichia coli, Serratia marcescens, Citrobacter freundii, Enterobacter cloacae, K. pneumoniae carrying blaKPC, blaNDM, and/or blaOXA-48. On the other hand, the assay did not exhibit any cross-reaction in control isolates harboring blaIMP and blaVIM. We conclude that the RESIST-3 O.K.N. K-SeT is a reliable, rapid, and user friendly test and we recommend it for routine diagnostic laboratories.

Keywords
Klebsiella pneumoniae, Immunochromatographic test, NDM, KPC, OXA-48
Braz J Microbiol 2018;49:885-90 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.002
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