Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:757-69 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.012
Environmental Microbiology
Petroleum contamination and bioaugmentation in bacterial rhizosphere communities from Avicennia schaueriana
Simone Dealtrya,b,, , Angela Michelato Ghizelinib,c, Leda C.S. Mendonça-Haglerb,c, Ricardo Moreira Chaloubd, Fernanda Reinertb,e, Tácio M.P. de Camposa, Newton C.M. Gomesf, Kornelia Smallag
a Pontifícia Universidade Católica do Rio de Janeiro, Departamento de Engenharia Civil, Rio de Janeiro, RJ, Brazil
b Universidade Federal do Rio de Janeiro, Programa de Pós-Graduação em Biotecnologia Vegetal, Rio de Janeiro, RJ, Brazil
c Universidade Federal do Rio de Janeiro, Instituto de Microbiologia Paulo de Góes, Rio de Janeiro, RJ, Brazil
d Universidade Federal do Rio de Janeiro, Instituto de Química, Departamento de Bioquímica Rio de Janeiro, RJ, Brazil
e Universidade Federal do Rio de Janeiro, Instituto de Biologia, Laboratório de Fisologia de Planta, Rio de Janeiro, RJ, Brazil
f Universidade de Aveiro, Departamento de Biologia, Centro de Estudos do Ambiente e do Mar (CESAM), Aveiro, Portugal
g Julius Kühn-Institut (JKI) Bundesforschungsinstitut für Kulturpflanzen, Institut für Epidemiologie und Pathogendiagnostik, Braunschweig, Germany
Received 12 May 2017, Accepted 14 February 2018
Abstract

Anthropogenic activity, such as accidental oil spills, are typical sources of urban mangrove pollution that may affect mangrove bacterial communities as well as their mobile genetic elements. To evaluate remediation strategies, we followed over the time the effects of a petroleum hydrocarbon degrading consortium inoculated on mangrove tree Avicennia schaueriana against artificial petroleum contamination in a phytoremediation greenhouse experiment. Interestingly, despite plant protection due to the inoculation, denaturing gradient gel electrophoresis of the bacterial 16S rRNA gene fragments amplified from the total community DNA indicated that the different treatments did not significantly affect the bacterial community composition. However, while the bacterial community was rather stable, pronounced shifts were observed in the abundance of bacteria carrying plasmids. A PCR-Southern blot hybridization analysis indicated an increase in the abundance of IncP-9 catabolic plasmids. Denaturing gradient gel electrophoresis of naphthalene dioxygenase (ndo) genes amplified from cDNA (RNA) indicated the dominance of a specific ndo gene in the inoculated petroleum amendment treatment. The petroleum hydrocarbon degrading consortium characterization indicated the prevalence of bacteria assigned to Pseudomonas spp., Comamonas spp. and Ochrobactrum spp. IncP-9 plasmids were detected for the first time in Comamonas sp. and Ochrobactrum spp., which is a novelty of this study.

Keywords
Phytoremediation, Mangrove, IncP-9 plasmids, ndo genes
Braz J Microbiol 2018;49:757-69 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.02.012
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