Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:865-71 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.03.009
Biotechnology and Industrial Microbiology
Statistical optimization of kojic acid production by a UV-induced mutant strain of Aspergillus terreus
Mojtaba Shakibaiea,b, Atefeh Americ,, , Roya Ghazanfariand, Mahboubeh Adeli-Sardoua, Sahar Amirpour-Rostamic, Masoud Torkzadeh-Mahanie, Mehdi Imanif, Hamid Forootanfarb,c,**,
a Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran
b Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
c Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran
d The Student Research Committee, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran
e Department of Biotechnology, Research institute for Science and High Technology and Environmental Sciences, Graduated University of Advanced Technology, Kerman, Iran
f Department of Basic Sciences, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran
Received 29 September 2017, Accepted 21 March 2018
Abstract

The ability of four Aspergillus strains for biosynthesis of kojic acid was evaluated among which Aspergillus terreus represented the highest level (2.21g/L) of kojic acid production. Improvement kojic acid production ability of A. terreus by random mutagenesis using different exposure time to ultraviolet light (5–40min) was then performed to obtain a suitable mutant of kojic acid production (designated as C5-10, 7.63g/L). Thereafter, design of experiment protocol was employed to find medium components (glucose, yeast extract, KH2PO4 (NH4)2SO4, and pH) influences on kojic acid production by the C5-10 mutant. A 25−1 fractional factorial design augmented to central composite design showed that glucose, yeast extract, and KH2PO4 were the most considerable factors within the tested levels (p<0.05). The optimum medium composition for the kojic acid production by the C5-10 mutant was found to be glucose, 98.4g/L; yeast extract, 1.0g/L; and KH2PO4, 10.3mM which was theoretically able to produce 120.2g/L of kojic acid based on the obtained response surface model for medium optimization. Using these medium compositions an experimental maximum Kojic acid production (109.0±10g/L) was acquired which verified the efficiency of the applied method.

Keywords
Kojic acid, Aspergillus terreus, UV mutagenesis, Medium optimization
Braz J Microbiol 2018;49:865-71 - Vol. 49 Num.4 DOI: 10.1016/j.bjm.2018.03.009
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