Brazilian Journal of Microbiology Brazilian Journal of Microbiology
Braz J Microbiol 2018;49:362-9 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.04.013
Biotechnology and Industrial Microbiology
The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi
Chang-hong Dinga, Qian-Bo Wangb, Shenglei Guoa, Zhen-yue Wanga,,
a Heilongjiang University of Chinese Medicine, Pharmacy College, Harbin, China
b The First Affiliated Hospital of Guangdong Pharmaceutical University, Department of Pharmacy, Guangzhou, China
Received 13 October 2016, Accepted 19 April 2017
Abstract

Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20d, and made spore concentration in co-culture medium was 1×104mL–1, after co-cultured for 12d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.

Keywords
Rumex gmelini Turcz (RGT), Endophytic fungi, Aspergillus sp., Seedlings, Bioactive secondary metabolites
Braz J Microbiol 2018;49:362-9 - Vol. 49 Num.2 DOI: 10.1016/j.bjm.2017.04.013
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